Stem Cell Disease Modelling Laboratory

The Stem Cell Disease Modelling Laboratory generates and characterises human stem cell lines for academic researchers and industry partners.

Stem Cell Disease Modelling Laboratory (SCDML) is a service node of Phenomics Australia. It provides on-demand reprogramming of patient samples into induced pluripotent stem cells (iPSCs), their quality control and banking, focusing on the production of large-scale iPSC cohort.

SCDML can reprogram from various primary cell types, and with different reprogramming approaches. Services include training of researchers in these procedures.

The platform is open to academic researchers and industry partners Australia-wide on a fee-for-service basis.

Services

• Reprogramming patient blood/skin samples using episomal vectors to generate polyclonal iPSCs
• Monoclonal lines can be generated on request
  • Quality control assessment of generated lines:
  • analysis of pluripotency markers (immunocytochemistry)
  • analysis of tri-lineage differentiation ability (immunocytochemistry)
  • analysis of colony morphology
  • mycoplasma testing
  • karyotyping (DNA extraction for SNP array)
  • STR analysis can be arranged on request
• Expanding the reprogrammed lines for 8 passages
• Supply 10 vials per reprogrammed line (5 at p0/p1, 5 at p8)
• PBMC isolation can be provided on request
• Tailored training in cell culture techniques for researchers entering the field
• Expansion of existing iPSC lines to share with other researchers
• Assistance with experimental planning
• Assistance with budget preparation for funding applications
• Assistance with amending ethics applications for funding applications
• Assistance with preparation of material transfer agreement documentation

Contact Us

Dr Maciej Daniszewski
maciej.daniszewski@unimelb.edu.au
+61 3 8344 8179

Visit  SCDML