Learn how to perform a range of techniques, including processing gel spots, digesting proteins in solution, and confirming chemical synthesis at the Mass Spectrometry and Proteomics Facility.
At the Mass Spectrometry and Proteomics Facility at the Bio21 Institute we teach academic and industry researchers how to perform their own mass spectrometry experiments using our instruments.
We have supported people to:
- Process their own gel spots
- Characterise intact recombinant proteins by mass
- Confirm a chemical synthesis
- Identify phosphorylation sites
- Perform immune-precipitation of protein complexes
- Perform both labelled and label-free quantitation experiments.
We help researchers identify proteins, quantify changes in protein expression levels and characterise post-translational modifications. We can perform:
- Quality control (QC) of chemical synthesis
- Recombinant protein QC
- Phosphoproteomics
- Label-free quantitation
- Tandem mass tag (TMT) quantitation
- Protein sequencing by mass spectrometry.
Resources
- 2 x Thermo OrbiTrap Fusion Lumos
- 2 x Thermo OrbiTrap Q Exactive Plus mass spectrometer
- Thermo OrbiTrap Exploris 480 mass spectrometer
- Thermo OrbiTrap Eclipse mass spectrometer
- Thermo OrbiTrap Ascend mass spectrometer
- Thermo OrbiTrap Exactive Focus mass spectrometer
- Agilent 6520 LC/ESI-TOF mass spectrometer
- Waters XSIMS QTOF mass spectrometer
- Waters HDX system inc. Synapt mass spectrometer
- Bruker TIMSTOF Pro mass spectrometer
- Shimadzu 8050 QQQ mass spectrometer
- Four Agilent HPLC systems for sample preparation
- Three Shimadzu Prep HPLC with UV and MS detection.
User information
The platform is open to all researchers open access via a subscription model. Please contact us for more information (see below).
Other platforms
The platform can be used in conjunction with:
Contact us
- Nick Williamson
- nawill@unimelb.edu.au
- Phone
- +61 3 8344 2206
Book online using iLab
You can book this resource or platform using the iLab booking system. You will need to create an account before logging in. Access user guides and videos to help you set up an account and navigate the iLab system:
If you're familiar with iLab and already have a login, visit the Mass Spectrometry and Proteomics Facility iLab Core.
More information on this platform can be found on the Research Gateway, which is available to all University of Melbourne academic and honorary staff, graduate researchers and professional staff. To access the Research Gateway, login with your University of Melbourne username and password.