The Melbourne Cytometry Platform offers equipment and expertise for analysing the physical and chemical characteristics of particles, cells and subcellular components.
The platform provides researchers access to flow cytometry for detecting, identifying, counting and isolating particles and cells, and also identifying molecular components within them. Cells or particles are suspended in fluid and then injected into a flow cytometer at high speed, passing one-by-one through focussed laser beams for analysis.
Our network of facilities on campus includes physical containment level 2 and level 3 facilities. We work with researchers from a broad range of disciplines, including nanotechnology, chemistry, cell biology, immunology, microbiology, developmental biology, bioengineering, biochemistry, oncology, medicine, neurosciences and material sciences.
We provide expert advice and advanced training on:
- Cytometry applications
- Assay development
- Experimental design and sample preparation
- Fluorescence panel design
- Instrument operation
- Data analysis
- Advanced instrument characterisation
- Optimum setup and fine tuning for advanced applications.
We collaborate with both academic researchers and industry partners. In 2019, the platform provided over 27 000 hours of services to 364 users, underpinning 73 research publications. We can cover a broad range cytometry challenges, from small particles and very dim signals to high dimensional multiparametric analysis.
- 2 Cytek Aurora 5L (5 lasers, 64 detectors)
- 3 Beckman Coulter CytoFLEX S & LX
- 6 Becton Dickinson LSR Fortessa
- Becton Dickinson LSR II, Canto II & FACSVerse
High-Speed Cell Sorters
- 2 Beckman Coulter AstriosEQ cell sorters (4 lasers, 16 detectors)\
- 2 Becton Dickinson FACS Aria III (4-5 lasers, 18 detectors)
- 1 Becton Dickinson FACS Aria Fusion (4 lasers, 16 detectors)
The platform is open to researchers Australia-wide on a fee-for-service basis.
The platform can be used in conjunction with the:
- Biological Optical Microscopy Platform
- Metabolomics Australia
- Melbourne Protein Characterisation
- Materials Characterisation and Fabrication Platform
- Biosciences Microscopy Unit
- Melbourne Advanced Microscopy Facility
- Australian Phenomics Network Histopathology and Slide Scanning Services
- Melbourne Histology Platform
- Melbourne Bioresources Platform.
Image: Alexis Gonzalez
Alexis Perez Gonzalez
Phone: +61 3 9341 1567
More information on this platform can be found on the Research Gateway, which is available to all University of Melbourne academic and honorary staff, graduate researchers and professional staff. Please note, to access the Research Gateway, you will need to login with your University of Melbourne username and password.