Mass Spectometry and Proteomics Facility

2 minute read

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Learn how to perform a range of techniques including processing gel spots, in solution protein digests and confirming a chemical synthesis at the Mass Spectrometry and Proteomics Facility.


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At the Mass Spectrometry and Proteomics Facility at the Bio21 Institute we teach academic and industry researchers how to perform their own mass spectrometry experiments using our instruments.

We have supported people to:

  • Process their own gel spots
  • Characterise intact recombinant proteins by mass
  • Confirm a chemical synthesis
  • Identify phosphorylation sites
  • Perform immune-precipitation of protein complexes
  • Perform both labelled and label-free quantitation experiments.

We help researchers identify proteins, quantify changes in protein expression levels and characterise post-translational modifications. We can perform:

  • Quality control (QC) of chemical synthesis
  • Recombinant protein QC
  • Phosphoproteomics
  • Label-free quantitation
  • Tandem mass tag (TMT) quantitation
  • Protein sequencing by mass spectrometry.

Resources

  • 2 x Thermo OrbiTrap Fusion Lumos
  • 2 x Thermo OrbiTrap Q Exactive Plus Mass spectrometer
  • Thermo OrbiTrap Exploris 480 mass spectrometer
  • Thermo OrbiTrap Eclipse mass spectrometer
  • Thermo OrbiTrap Exactive Focus mass spectrometer
  • Agilent 6520 LC/ESI-TOF mass spectrometer
  • Waters Vion IMS QTOF Mass spectrometer
  • Waters HDX system inc. Synapt mass spectrometer
  • Four Agilent HPLC systems for sample preparation.
  • Three Shimadzu Prep HPLC with UV and MS detection

User information

The platform is open to all researchers open access via a subscription model. Please contact us for more information (see below).

Other platforms

The platform can be used in conjunction with:

Image: Peter Casamento.

Contact us

Nick Williamson

nawill@unimelb.edu.au

Phone: +61 3 8344 2206

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More information on this facility can be found on the Research Gateway, which is available to all University of Melbourne academic and honorary staff, graduate researchers and professional staff.  Please note, to access the Research Gateway, you will need to login with your University of Melbourne username and password.